Test&Treat Ltd

Technical support

U-treat technology is intended for veterinary use only for the testing of urine samples in dogs and cats. U-treat reliably detects the presence of bacteria in urine and provides quantitative antibiotic sensitivity and resistance profiles.

Used alongside your routine urinalysis procedures, it’s the complete solution for your in-clinic urinalysis needs.

TEST 1: Identify a positive/negative result

Step 1

Allow the Reagent 1 tube (R1) to reach 37˚C and the Reagent 2 device (R2) to come to room temperature (around 15 minutes).

Step 2

Turn on the Luminometer and update the time/date if needed.

Step 3

When the R1 tube has reached 37˚C and the R2 device has reached room temperature, add 25 microlitres of your sample to the R1 tube and incubate in the Dry Bath Incubator at 37°C for five minutes.

Step 4

Press the test button (test tube icon) on the Luminometer and select ‘Test 1’ using the green tick icon.

Step 5

Once incubation is complete, screw the R2 device onto the top of the R1 tube and turn the device plunger anti-clockwise to unlock. Push the plunger down to release the contents into the R1 tube and gently agitate to mix thoroughly.

Step 6

Open the Luminometer lid and immediately place the R1 tube (with R2 device attached) inside. Close the lid and press the start button (small play triangle on the right-hand side) to begin.

Results

You’ll see a positive or negative result within one minute. 5,000 RLUs and above is a positive UTI result. Below 5,000 RLUs is a negative UTI result. You should follow a result of 5,000-20,000 RLUs by repeating Test 1. When you’ve confirmed a positive result, move onto Test 2.

TEST 2: Identify antibiotic susceptibility

Step 1

Allow your R1 tubes to reach 37˚C and R2 devices to come to room temperature (you can test up to six antibiotics plus a control tube at one time).

Step 2

When the R1 tubes have reached 37˚C and the R2 devices have reached room temperature, add 25 microlitres of your sample to the R1 tubes and pre-incubate in the Dry Bath Incubator at 37˚C for five minutes.

Step 3

Press the test button (test tube icon) on the Luminometer and select ‘Test 2’ using the green tick icon.

Step 4

Once pre-incubation is complete, assign an antibiotic to each R1 tube (except the control tube) and label as channel 1, 2, 3 etc. Add your chosen antibiotics to the assigned R1 tubes and incubate for 30 minutes in the Dry Bath Incubator.

Step 5

Once incubation is complete, screw the R2 device onto the control tube and turn the device plunger anti-clockwise to unlock. Then depress the plunger to mix the reagents. *If using Trimethorpin Sulfamethoxazole, you MUST incubate it for 90 minutes and run the process separately with its own control tube (alternatively run all tubes for 90 minutes).

Step 6

Immediately place the control tube into the Luminometer (after selecting ‘Test 2’) and press the green arrow icon. Repeat this process for each R1 tube you’ve mixed with antibiotics.

Results

Press the results icon to access the results for Test 2. The numbers relate to your channel labels (with ‘C’ being the control tube).

The Luminometer display will show the percentage reduction of signal for each antibiotic compared to the control. This will show you the % kill rate that each antibiotic has against this particular UTI, helping you to choose which to prescribe.

Troubleshooting

Should you have any issues using U-treat, please refer to our Troubleshooting Guide.

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